本书涵盖了元基因文库的构建、元基组文库的筛选技术和针对不同活性目标产物的筛选方法，并且提供了详细实用的实验操作方法，可以帮助希望从事元基因组学研究的科技人员尽快建立相关的实验平台。
本书适合于从事环境微生物学、微生物生态学等相关专业的高年级本科生、研究生，以及相关研究工作者等参考使用。
本书涵盖了元基因组文库的构建、元基因组文库的筛选技术和针对不同活性目标产物的筛选方法, 并且提供了详实实用的实验操作方法。
Preface
Contributrs
1 Molecular Methods to Study Complex Microbial Communities jose M.Vieites,Mar韆-Eugenia Guazzaroni,Ana Beloqui,Peter N.Golyshin,andManuel Ferrer
2 Contruction of Small-Inert and Large-Inert Metagenomic Libraries Carola Simon and Rolf Daniel
3 Contruction and Screening of Marine Metagenomic Libraries Nancy Weiland,Carolin Loscher,Rebekka Metzger,and Rutb Schmitz
4 Metagenomic Analysis of Isotopically Enriched DNA Rin Chen,josh D.Neufeld,Marc G.Dumont,Michael W.Friedrich,and J.Colin Murrell
5 Wide Host-Range Cloning for Functional Metagenomics Michael W.Friedrich Margaret Wexler and Andrew W.B. Johnston
6 Cloning and Expression Vecton for a Gram-Positive Host, Streptomyces lividans Kristof Vrancken,Lieve Van Mellaert,and Jozef Anne
7 Heterologeus Gene Expression in the Hyperthermophilic Archaeon Sulfolobus solfataricus Angel Angelov and Wolfgang Liebl
8 Novel Tools for the Functional Expression of Metagenomic DNA Sonja Christina Troeschel,Thomas Drepper,Christian Leggewie,Wlfgang R.Streit,and Karl-Erich Jaeger
9 Screening of Functional Promoter from Metagenomic DNA for Practical Use in Expression Systems So-Roun Park and Geun-Joong Kim
10 Substrate-Induced Gene Expression Screening: A Method for High-Throughput Screening of Metagenome Libraries taku Uchiyama and Kentaro Miyazaki
11 Screen for Active and Stereoselective Hydrolytic Enzymes Dominique Bottcher,Marlen Schmaidt,and Uwe T.Bornscheuer
12 Screening for Cellulase-Encoding Clones in Metagenomic Libraries Nele Ilmberger and Wlfgang R.Streit
13 Screening Metagenomic Libraries for Laccase Activities Manuel Ferrer,Ana Belqui,and Peter N.Golyshin
14 Screening for N-AHSL-Based-Signaling Interfering Enzymes Phil M.Oger and Stephane Uroz
15 Identification of Molecular Marken to Follow Up the Bioremediation of Sites Contaminated with Chlorinated Compounds Massimo Marzorati,Annalisa Balloi,Francesca De Ferra,and Daniele Ddaffonchio
16 Methods for the Isolation of Genes Encoding Novel PHB Cycle Enzymes from Complex Microbial Communities Ricardo F.Nordeste,Maria A.trainer,and Trevor C.Charles
17 Metagenomic Approaches to Identify and Isolate Bioactive Natural Products from Microbiota of Marine Sponges Cristian Gurgui and Jorn Piel
18 Screening for Novel Antibiotic Resistance Genes Mindy G.Brown,Elizabeth H.Mitchell,andDavid L.Balkwill
19 Novel Metal Resistance Genes from Microorganisms:A Functional Metagenomic Approach Jose E.Gonzalez-Pastor and Salvador Mirete
20 Retrieval of Full-Length Functional Genes Using Subtractive Hybridization Magnetic Bead Capture Tracy Meiring,Inonge Mulako,Marla I.Tuffin,Quinton Meyer,and Donald A.Cowan
21 Detection and Isolation of Selected Genes of Interest from Metagenomic Libraries by a DNA Microarray Approach Gopal P.Pathak and Wlfgang Gartner
22 Application ofDNA Microarray for Screening Metagenome Library Clones Soo-Je Park,Jong-Chan Chae,and Sung-Keun Rhee
23 MetaGenomeThreader:A Software Tool for Predicting Genes in DNA-Sequences of Metagenome Projects David J.Scbmitz-Hubsch and Stefan Kurtz
Index
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