The present book is the second version revised from CellEngineering (Second Edition), and was selected as a nationalteaching material for Chinese higher education in the 11t" Five- Year Plan. 《Cell Engineering(Second Edition)》包括：comprises three parts: fundamentals of cellengineering, plant cell engineering, and animal cell engineering.Every effort has been made to comprehensively, systematicallyand concisely present the principles as well as techniques inrelation to cell engineering. It is not only suitable as a text bookfor undergraduates majoring in bioengineering, biotechnology,medicine and pharmacy, agriculture etc, but also suitable as areference book for cell engineering researchers

PrefacePart 1  Fundamentals of Cell EngineeringChapter 1  Introduction to Cell Engineering  1.1  Bioengineering  1.2  Cell EngineeringChapter 2  Cell  PrefacePart 1 Fundamentals of Cell EngineeringChapter 1 Introduction to Cell Engineering 1.1 Bioengineering 1.2 Cell EngineeringChapter 2 Cell Engineering Basics 2.1 Composition of Cells 2.2 Cell Cycle & Division 2.3 Cell Apoptosis & Cytoclasis 2 4 Differentiation & Dedifferentiation 2.5 Tissue &Organ 2.6 Reproduction &DevelopmentPart 2 Plant Cell EngineeringChapter 3 Plant Tissue & Organ Culture 3.1 Plant Tissue Culture 3.2 Plant Embryo Culture 3.3 Hairy Root CultureChapter 4 Artificial Seeds & Virus-Free of Plants 4.1 Artificial Seeds 4.2 Virus Elimination of PlantsChapter 5 Plant Cell Culture & Preparation of Secondary Metabolites 5.1 Plant Cell Culture and Its Characteristics 5.2 Plant Cell Culture Techniques 5.3 Production of Secondary Metabolites by Plant Cell CultureChapter 6 Protoplast Culture & Mutagenesis 6.1 Protoplast Culture 6.2 Protoplast MutagenesisChapter 7 Cell Fusion & Somatic Cell Hybridization 7 I Cell Fusion 7.2 Somatic HybridizationChapter 8 Polyploid and Haploid Plants 8.1 Chromosome Engineering 8.2 Polyploid Plants 8.3 Haploids and Homozygous Diploid PlantsChapter 9 In Vitro Fertilization 9.1 Plant in Vitro Fertilization 9.2 In Vitro Pollination 9.3 In Vitro FertilizationChapter 10 Transgenic Techniques 10.1 Transgenic Plants 10.2 Genetically Modified Plants 10.3 Biopharmaceutical Transgenic Plants 10.4 Safety of Transgenic PlantsPart 3 Animal Cell EngineeringChapter 11 Animal Cell Culture & Preparation and Expression of Medicinal Proteins 11.1 Animal Cell Culture 11.2 Characteristics of Animal Cell Culture 11.3 Culture Tools and Conditions 11.4 Growth of in Vitro Cultured Cells 11.5 Cell Line/Strain and Preservation 11.6 Small-Scale Animal Cell Culture Methods 11.7 Large-Scale Animal Cell Culture 11.8 Animal Cell Culture Bioreactors 11.9 Factors of Large-Scale Animal Cell Culture 11.10 Preparation of Pharmaceutical Proteins with Animal CellsChapter 12 Hybridoma Technology and Monoclonal Antibody 12.1 Hybridoma Technology 12.2 Monoclonal Antibody 12.3 Humanized Monoclonal AntibodyChapter 13 In Vitro Fertilization & Animals Cloned by Nuclear Transfer 13.1 Embryo Technique 13.2 IVF Animals 13.3 Test-Tube Baby 13.4 Animals Cloned by Nuclear Transfer 13.5 Cryopreservation Technique 13.6 Mammalian Sex ControlChapter 14 Polyploid and Transgenic Animals 14.1 Polyploid Animals 14.2 Transgenic AnimalsChapter 15 Stem Cells 15.1 Characteristics of Stem Cells 15.2 Embryonic Stem Cells 15.3 Adult Stem CellsChapter 16 Tissue Engineering 16.1 Tissue Engineering and Its Three Elements 16.2 Technical Routes of Tissue Engineering 16.3 Tissue Engineering Bioreactors 16.4 Tissue Engineering Products Index